Center for microbial life detection at Medical University Graz, Austria
ZMF - Center for Medical Research
The “center for life detection” will provide expertise in detection of microbial signatures, analysis thereof and microbial cultivation. The involved members have large experience with microbial detection and quantification in samples from extreme environments and the growth of microbial specialists in (pure) cultures (e.g. anaerobes). Specifically we offer:
• Life detection in environmental and appropriate clinical samples (support in DNA extraction, selection of appropriate primers for bacteria, archaea and fungi, polymerase chain reaction (PCR), if desired in combination with propidium monoazide staining (detection of intact cells only), amplicon-sequencing and diversity data analysis, quantification of bacteria, archaea and fungi. If needed, -OMICS technologies can be applied.
• For the life detection workflow e.g. Next Generation Sequencing (Illumina MiSeq) for nucleic acid characterization, Gas Chromatography - Mass Spectrometry (GC-MS) for short fatty acids determination or scanning electron microscope SEM ZEISS DSM 950 for ultrastructure analysis.
• Detection of microbial cells: Domain to genus-specific fluorescence in situ hybridization, probe design and selection, visualization using confocal laser scanning microscopy (CLSM).
• Cultivation of specific microbial specialists, such as anaerobes or oligotrophs for use in laboratory experiments at the host’s institution.
• Support in data analysis includes graphical display of e.g. microbial diversity and interpretation of results with respect to the metabolic capabilities of the microbiome and the possible impact on the habitat.
All services will be offered to international users and all ZMF Core Facilities are open to all researchers without restrictions. The ZMF and IMRG staff will support users with protocol development, hand-on training and analyses.
For further information contact: Prof. Christine Moissl-Eichinger, Department of Internal Medicine